Leptospirosis occurs worldwide and is a common, mild to severe health problem for humans and animals, particularly in areas with hot and humid climates. Leptospirosis is often overlooked since it mimics many other diseases, and despite its high fatality rate, leptospirosis is curable with early treatment. Thus, accurate and rapid diagnosis is important. Laboratory tests are, therefore, the basis for diagnosis of leptospirosis; the most commonly used laboratory tests are based on detection of antibodies against the leptospires. A rapid screening test result allows for presumptive diagnosis that can then be confirmed by other diagnostic methods including culture, microscopic agglutination test (MAT) and ELISA., Despite its high fatality rate, leptospirosis is curable with early treatment, thus, accurate and rapid diagnosis is important.