World under LOCKDOWN (COVID-19)
On December 31 of 2019, Chinese authorities alerted the World Health Organization of an outbreak of a novel strain of coronavirus causing severe illness, which was subsequently named SARS-CoV-2. Breaking all boundaries, this virus within 2 months had spread almost all over the world and on March 11 of 2020, WHO declared that COVID-19 can be characterized as a pandemic.
COVs are enveloped viruses with a positive-sense single-stranded RNA genome and a nucleocapsid of helical symmetry. The genome size of corona viruses ranges from approximately 27 to 34 kilobases, the largest among known RNA viruses.
Symptoms: The COVID-19 virus affects different people in different ways. Common symptoms include fever, tiredness and dry cough. Other symptoms include shortness of breath, aches and pains, sore throat and very few people will report diarrhea, nausea, a runny nose and loss of taste or smell. Older people, and those with underlying medical problems like cardiovascular disease, diabetes, chronic respiratory disease, and cancer are more likely to develop serious illness
This virus spreads primarily through droplets generated when an infected person coughs or sneezes, or through droplets of saliva or discharge from the nose. To protect yourself, clean your hands frequently with an alcohol-based hand rub or wash them with soap and water. Wearing mask and social distancing seems to be the only solution.
Athenese Products -Joins the fight against CORONA
ADx Hand Sanitizer:
At present, alcohol-based hand rubs are the only known means for rapidly and effectively inactivating a wide array of potentially harmful microorganisms on hands. ADx Hand sanitizers is based on WHO recommended formula
• Ethanol based formula – Ethanol denatured 80% v/v, Glycerol 1.45% v/v, Hydrogen peroxide (H2O2) 0.125% v/v
• Isopropanol based formula -Isopropyl alcohol 75% v/v, Glycerol 1.45% v/v, Hydrogen peroxide (H2O2) 0.125% v/v
The Alcohol percentage kills 99.99% of microbes, addition of Hydrogen peroxide eliminates contaminating spores and Glycerol is added as a humectant to increase the acceptability of the product.
It quickly measures body temperature without making any contact with a person’s skin and has become an important tool for countries scrambling to contain viral outbreaks as fever is one of the most common symptoms in such cases. Athenese IR thermometer comes with dual mode option (Centigrade and Fahrenheit), records temperature within a second from a distance of 2.5 cm. It has a distinctly audible alarm, excellent measuring range with a resolution of 0.1°C and above all comes with 2 years warranty.
Athenese in Diagnosing Corona
Experts have said comprehensive testing is key to controlling coronavirus, and creating a more accurate picture of the spread of Covid-19.Testing will help – to diagnose them individually and to work out how far the virus has spread. This information will help the health service to plan better.
In the analytic stage, Real Time -Polymerase Chain Reaction (RT-PCR) assays remain the molecular test of choice for the etiologic diagnosis of SARS -CoV -2 infection while antibody -based techniques are being introduced as supplemental tools or screening.
With full genome of the virus being reported by scientist in China, molecular test for COVID 19 became feasible all over the world. The PCR test “detect the genetic information of the virus, the RNA. That’s only possible if the virus is there and someone is actively infected.”
Technician isolates the genetic material of the virus from a nasopharyngeal sample (obtained from a person by a swab of the upper respiratory tract).
Transport of Sample to diagnosis center
Viral Transport medium (VTM) is a specially formulated medium for collection and transport of viruses. It is designed to maintain the optimum viability and virulence of the viral sample.
Aridia Viral Transport Kit
Aridia Viral Transport Medium is made of Hanks’ Balanced Salt Solution and contains a protective protein antibiotic to control microbial contamination and buffers to control the pH.
The samples are further processed to remove the RNA of COVID 19 virus.
RNA Extraction The process of RNA extraction can be done manually as well as by using automated instruments. Athenese provides its customer with both manual and automated viral DNA/RNA extraction kit approved by ICMR with 100% concordance with True positive and True negative samples.
Manual Method-Aridia® DNA/RNA Complete Extraction kit (Spin column Method)
It offers a highly efficient, fast and simultaneous purification of viral DNA and RNA from cell–free samples such as serum, plasma and swab. The kit uses advance technology based on nucleic acid ability to bind silica in the presence of high concentrations of chaotropic salts. The viral DNA/RNA molecules bind to the silica-based media and impurities such as proteins and nucleases are removed by washing with Wash Buffer. The DNA/RNA is then eluted by Elution buffer in sterile, Elution Buffer or RNase free water.
Automatic Method -Aridia® DNA/RNA Complete Extraction kit (Magnetic bead Method)
Automatic Aridia extraction kit ( 64 test/pack ) consists of 4 plates of 96 well each.16 patient samples can be processed in single plate. To extract RNA in purified form each sample is processed in 6 wells. Lysis buffer, Magnetic bead, wash buffer I and wash buffer II, empty well (to avoid contamination) and elution buffer are systematically arranged in these wells and the extraction is done by using magnetic beads. This Kit is compatible with closed system ALTA Nucleic acid extractor wherein 2 plates (32 samples) can be processed at a time.
ALTA Nucleic acid extractor:
This CE marked; closed system compatible with Athenese Aridia kit can be easily operated through a 3.5” touch screen in combination with an unmatched remote-control Pad. It utilizes the proven magnetic field technology to extract highly purified Nucleic acid. With the convenient software based on Android, a protocol can be set up and started with just a few clicks on the pad. Friendly and intuitive interface make it simple even for first time user. It offers high throughput of 32 sample per run with two extraction kit run at a time.
VIASURE SARS-CoV-2 Real Time PCR Detection Kit -Approved by CE IVD
VIASURE SARS-CoV-2 Real Time PCR Detection Kit is designed for the diagnosis of SARS-CoV-2 in respiratory samples. The detection is done in one step real time RT format where the reverse transcription and the subsequent amplification of specific target sequence occur in the same reaction well. The isolated RNA target is transcribed generating complementary DNA by reverse transcriptase which is followed by the amplification of a conserved region of ORF1ab and N genes for SARS-CoV-2 using specific primers and a fluorescent-labeled probe.
VIASURE SARS-CoV-2 Real Time PCR Detection Kit contains in each well all the components necessary for real time PCR assay (specific primers/probes, dNTPS, buffer, polymerase and retro transcriptase) in a stabilized format, as well as an internal control to monitor PCR inhibition. ORF1ab gene is amplified and detected in FAM channel, N gene is amplified and detected in ROX channel and the internal control (IC) in HEX channel, VIC or JOE channel
VIASURE SARS-CoV-2 Real Time PCR Detection Kit has a detection limit of ≥
10 RNA copies per reaction for ORF1ab and N genes
Real Time PCR system ALTA RT-48
The ALTA RT-48 is a Quantitative, real-time PCR system providing precise and accurate results and has been widely used in corona test center for COVID 19 confirmation.
Highlights of the Instrument:
- Design for flexible use
- Fast and uniform heating System
- Highly sensitive optical system
- Easy-to-use Software
- Simultaneously detect 48 samples .
- Sample dynamic range could be from 101 to 1010 copies
- Repeatability: CV≤0.5%
- Stores over 1000 experiment setting / experiment data files
- Convenient loading platform, concealed hot lid ensuring operation safety and configuration setting &data safety during power off
Athenese staff at work -Installing ALTA Nucleic acid extractor and ALTA RT -48 instruments at COVID Test Centers